@article{He-2018-Misfolding,
title = "Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium",
author = "He, Yanping and
Chen, Da and
Huang, Po‐Jung Jimmy and
Zhou, Yibo and
Ma, Lingzi and
Xu, Kexin and
Yang, Ronghua and
Liu, Juewen",
journal = "Nucleic Acids Research, Volume 46, Issue 19",
volume = "46",
number = "19",
year = "2018",
publisher = "Oxford University Press (OUP)",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G18-52001",
doi = "10.1093/nar/gky807",
pages = "10262--10271",
abstract = "Herein, the excellent Na+ selectivity of a few RNA-cleaving DNAzymes was exploited, where Na+ can be around 3000-fold more effective than K+ for promoting catalysis. By using a double mutant based on the Ce13d DNAzyme, and by lowering the temperature, increased 2-aminopurine (2AP) fluorescence was observed with addition of both Na+ and K+. The fluorescence increase was similar for these two metals at below 10 mM, after which K+ took a different pathway. Since 2AP probes its local base stacking environment, K+ can be considered to induce misfolding. Binding of both Na+ and K+ was specific, since single base mutations could fully inhibit 2AP fluorescence for both metals. The binding thermodynamics was measured by temperature-dependent experiments revealing enthalpy-driven binding for both metals and less coordination sites compared to G-quadruplex DNA. Cleavage activity assays indicated a moderate cleavage activity with 10 mM K+, while further increase of K+ inhibited the activity, also supporting its misfolding of the DNAzyme. For comparison, a G-quadruplex DNA was also studied using the same system, where Na+ and K+ led to the same final state with only around 8-fold difference in Kd. This study provides interesting insights into strategies for discriminating Na+ and K+.",
}
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<abstract>Herein, the excellent Na+ selectivity of a few RNA-cleaving DNAzymes was exploited, where Na+ can be around 3000-fold more effective than K+ for promoting catalysis. By using a double mutant based on the Ce13d DNAzyme, and by lowering the temperature, increased 2-aminopurine (2AP) fluorescence was observed with addition of both Na+ and K+. The fluorescence increase was similar for these two metals at below 10 mM, after which K+ took a different pathway. Since 2AP probes its local base stacking environment, K+ can be considered to induce misfolding. Binding of both Na+ and K+ was specific, since single base mutations could fully inhibit 2AP fluorescence for both metals. The binding thermodynamics was measured by temperature-dependent experiments revealing enthalpy-driven binding for both metals and less coordination sites compared to G-quadruplex DNA. Cleavage activity assays indicated a moderate cleavage activity with 10 mM K+, while further increase of K+ inhibited the activity, also supporting its misfolding of the DNAzyme. For comparison, a G-quadruplex DNA was also studied using the same system, where Na+ and K+ led to the same final state with only around 8-fold difference in Kd. This study provides interesting insights into strategies for discriminating Na+ and K+.</abstract>
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%0 Journal Article
%T Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium
%A He, Yanping
%A Chen, Da
%A Huang, Po‐Jung Jimmy
%A Zhou, Yibo
%A Ma, Lingzi
%A Xu, Kexin
%A Yang, Ronghua
%A Liu, Juewen
%J Nucleic Acids Research, Volume 46, Issue 19
%D 2018
%V 46
%N 19
%I Oxford University Press (OUP)
%F He-2018-Misfolding
%X Herein, the excellent Na+ selectivity of a few RNA-cleaving DNAzymes was exploited, where Na+ can be around 3000-fold more effective than K+ for promoting catalysis. By using a double mutant based on the Ce13d DNAzyme, and by lowering the temperature, increased 2-aminopurine (2AP) fluorescence was observed with addition of both Na+ and K+. The fluorescence increase was similar for these two metals at below 10 mM, after which K+ took a different pathway. Since 2AP probes its local base stacking environment, K+ can be considered to induce misfolding. Binding of both Na+ and K+ was specific, since single base mutations could fully inhibit 2AP fluorescence for both metals. The binding thermodynamics was measured by temperature-dependent experiments revealing enthalpy-driven binding for both metals and less coordination sites compared to G-quadruplex DNA. Cleavage activity assays indicated a moderate cleavage activity with 10 mM K+, while further increase of K+ inhibited the activity, also supporting its misfolding of the DNAzyme. For comparison, a G-quadruplex DNA was also studied using the same system, where Na+ and K+ led to the same final state with only around 8-fold difference in Kd. This study provides interesting insights into strategies for discriminating Na+ and K+.
%R 10.1093/nar/gky807
%U https://gwf-uwaterloo.github.io/gwf-publications/G18-52001
%U https://doi.org/10.1093/nar/gky807
%P 10262-10271
Markdown (Informal)
[Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium](https://gwf-uwaterloo.github.io/gwf-publications/G18-52001) (He et al., GWF 2018)
ACL
- Yanping He, Da Chen, Po‐Jung Jimmy Huang, Yibo Zhou, Lingzi Ma, Kexin Xu, Ronghua Yang, and Juewen Liu. 2018. Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium. Nucleic Acids Research, Volume 46, Issue 19, 46(19):10262–10271.
