Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern
Opeyemi U. Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Sean Ma, Hanlan McDougall, R. Michael L. McKay, Aleksandra Mloszewska, Claire Oswald, Mark R. Servos, Megan Swinwood-Sky, Gustavo Ybazeta, Marc Habash, Lawrence Goodridge
Abstract
We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.- Cite:
- Opeyemi U. Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Sean Ma, Hanlan McDougall, R. Michael L. McKay, Aleksandra Mloszewska, Claire Oswald, Mark R. Servos, Megan Swinwood-Sky, Gustavo Ybazeta, et al.. 2022. Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern. Microbiology Resource Announcements, Volume 11, Issue 7, 11(7).
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@article{Lawal-2022-Metagenomics,
title = "Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern",
author = "Lawal, Opeyemi U. and
Zhang, Linkang and
Parreira, Valeria R. and
Brown, R. Stephen and
Chettleburgh, Charles and
Dannah, Nora and
Delatolla, Robert and
Gilbride, Kimberly and
Graber, Tyson E. and
Islam, Golam and
Knockleby, James and
Ma, Sean and
McDougall, Hanlan and
McKay, R. Michael L. and
Mloszewska, Aleksandra and
Oswald, Claire and
Servos, Mark R. and
Swinwood-Sky, Megan and
Ybazeta, Gustavo and
Habash, Marc and
Goodridge, Lawrence",
journal = "Microbiology Resource Announcements, Volume 11, Issue 7",
volume = "11",
number = "7",
year = "2022",
publisher = "American Society for Microbiology",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G22-84001",
doi = "10.1128/mra.00362-22",
abstract = "We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.",
}
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<abstract>We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.</abstract>
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%0 Journal Article %T Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern %A Lawal, Opeyemi U. %A Zhang, Linkang %A Parreira, Valeria R. %A Brown, R. Stephen %A Chettleburgh, Charles %A Dannah, Nora %A Delatolla, Robert %A Gilbride, Kimberly %A Graber, Tyson E. %A Islam, Golam %A Knockleby, James %A Ma, Sean %A McDougall, Hanlan %A McKay, R. Michael L. %A Mloszewska, Aleksandra %A Oswald, Claire %A Servos, Mark R. %A Swinwood-Sky, Megan %A Ybazeta, Gustavo %A Habash, Marc %A Goodridge, Lawrence %J Microbiology Resource Announcements, Volume 11, Issue 7 %D 2022 %V 11 %N 7 %I American Society for Microbiology %F Lawal-2022-Metagenomics %X We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples. %R 10.1128/mra.00362-22 %U https://gwf-uwaterloo.github.io/gwf-publications/G22-84001 %U https://doi.org/10.1128/mra.00362-22
Markdown (Informal)
[Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern](https://gwf-uwaterloo.github.io/gwf-publications/G22-84001) (Lawal et al., GWF 2022)
- Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern (Lawal et al., GWF 2022)
ACL
- Opeyemi U. Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Sean Ma, Hanlan McDougall, R. Michael L. McKay, Aleksandra Mloszewska, Claire Oswald, Mark R. Servos, Megan Swinwood-Sky, Gustavo Ybazeta, et al.. 2022. Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern. Microbiology Resource Announcements, Volume 11, Issue 7, 11(7).