@article{Abbes-2022-Occurrence,
title = "Occurrence of BMAA Isomers in Bloom-Impacted Lakes and Reservoirs of Brazil, Canada, France, Mexico, and the United Kingdom",
author = "Abbes, Safa and
Duy, Sung Vo and
Munoz, Gabriel and
Dinh, Quoc Tuc and
Simon, Dana F. and
Husk, Barry and
Baulch, Helen M. and
Vin{\c{c}}on‐Leite, Brigitte and
Fortin, Nathalie and
Greer, Charles W. and
Larsen, Megan L. and
Venkiteswaran, Jason J. and
Mart{\'\i}nez‐Jer{\'o}nimo, Fernando and
Giani, Alessandra and
Lowe, Chris and
Tromas, Nicolas and
Sauv{\'e}, S{\'e}bastien",
journal = "Toxins, Volume 14, Issue 4",
volume = "14",
number = "4",
year = "2022",
publisher = "MDPI AG",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G22-22001",
doi = "10.3390/toxins14040251",
pages = "251",
abstract = "The neurotoxic alkaloid β-N-methyl-amino-l-alanine (BMAA) and related isomers, including N-(2-aminoethyl glycine) (AEG), β-amino-N-methyl alanine (BAMA), and 2,4-diaminobutyric acid (DAB), have been reported previously in cyanobacterial samples. However, there are conflicting reports regarding their occurrence in surface waters. In this study, we evaluated the impact of amending lake water samples with trichloroacetic acid (0.1 M TCA) on the detection of BMAA isomers, compared with pre-existing protocols. A sensitive instrumental method was enlisted for the survey, with limits of detection in the range of 5-10 ng L-1. Higher detection rates and significantly greater levels (paired Wilcoxon's signed-rank tests, p {\textless} 0.001) of BMAA isomers were observed in TCA-amended samples (method B) compared to samples without TCA (method A). The overall range of B/A ratios was 0.67-8.25 for AEG (up to +725{\%}) and 0.69-15.5 for DAB (up to +1450{\%}), with absolute concentration increases in TCA-amended samples of up to +15,000 ng L-1 for AEG and +650 ng L-1 for DAB. We also documented the trends in the occurrence of BMAA isomers for a large breadth of field-collected lakes from Brazil, Canada, France, Mexico, and the United Kingdom. Data gathered during this overarching campaign (overall, n = 390 within 45 lake sampling sites) indicated frequent detections of AEG and DAB isomers, with detection rates of 30{\%} and 43{\%} and maximum levels of 19,000 ng L-1 and 1100 ng L-1, respectively. In contrast, BAMA was found in less than 8{\%} of the water samples, and BMAA was not found in any sample. These results support the analyses of free-living cyanobacteria, wherein BMAA was often reported at concentrations of 2-4 orders of magnitude lower than AEG and DAB. Seasonal measurements conducted at two bloom-impacted lakes indicated limited correlations of BMAA isomers with total microcystins or chlorophyll-a, which deserves further investigation.",
}
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<abstract>The neurotoxic alkaloid β-N-methyl-amino-l-alanine (BMAA) and related isomers, including N-(2-aminoethyl glycine) (AEG), β-amino-N-methyl alanine (BAMA), and 2,4-diaminobutyric acid (DAB), have been reported previously in cyanobacterial samples. However, there are conflicting reports regarding their occurrence in surface waters. In this study, we evaluated the impact of amending lake water samples with trichloroacetic acid (0.1 M TCA) on the detection of BMAA isomers, compared with pre-existing protocols. A sensitive instrumental method was enlisted for the survey, with limits of detection in the range of 5-10 ng L-1. Higher detection rates and significantly greater levels (paired Wilcoxon’s signed-rank tests, p \textless 0.001) of BMAA isomers were observed in TCA-amended samples (method B) compared to samples without TCA (method A). The overall range of B/A ratios was 0.67-8.25 for AEG (up to +725%) and 0.69-15.5 for DAB (up to +1450%), with absolute concentration increases in TCA-amended samples of up to +15,000 ng L-1 for AEG and +650 ng L-1 for DAB. We also documented the trends in the occurrence of BMAA isomers for a large breadth of field-collected lakes from Brazil, Canada, France, Mexico, and the United Kingdom. Data gathered during this overarching campaign (overall, n = 390 within 45 lake sampling sites) indicated frequent detections of AEG and DAB isomers, with detection rates of 30% and 43% and maximum levels of 19,000 ng L-1 and 1100 ng L-1, respectively. In contrast, BAMA was found in less than 8% of the water samples, and BMAA was not found in any sample. These results support the analyses of free-living cyanobacteria, wherein BMAA was often reported at concentrations of 2-4 orders of magnitude lower than AEG and DAB. Seasonal measurements conducted at two bloom-impacted lakes indicated limited correlations of BMAA isomers with total microcystins or chlorophyll-a, which deserves further investigation.</abstract>
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%0 Journal Article
%T Occurrence of BMAA Isomers in Bloom-Impacted Lakes and Reservoirs of Brazil, Canada, France, Mexico, and the United Kingdom
%A Abbes, Safa
%A Duy, Sung Vo
%A Munoz, Gabriel
%A Dinh, Quoc Tuc
%A Simon, Dana F.
%A Husk, Barry
%A Baulch, Helen M.
%A Vinçon‐Leite, Brigitte
%A Fortin, Nathalie
%A Greer, Charles W.
%A Larsen, Megan L.
%A Venkiteswaran, Jason J.
%A Martínez‐Jerónimo, Fernando
%A Giani, Alessandra
%A Lowe, Chris
%A Tromas, Nicolas
%A Sauvé, Sébastien
%J Toxins, Volume 14, Issue 4
%D 2022
%V 14
%N 4
%I MDPI AG
%F Abbes-2022-Occurrence
%X The neurotoxic alkaloid β-N-methyl-amino-l-alanine (BMAA) and related isomers, including N-(2-aminoethyl glycine) (AEG), β-amino-N-methyl alanine (BAMA), and 2,4-diaminobutyric acid (DAB), have been reported previously in cyanobacterial samples. However, there are conflicting reports regarding their occurrence in surface waters. In this study, we evaluated the impact of amending lake water samples with trichloroacetic acid (0.1 M TCA) on the detection of BMAA isomers, compared with pre-existing protocols. A sensitive instrumental method was enlisted for the survey, with limits of detection in the range of 5-10 ng L-1. Higher detection rates and significantly greater levels (paired Wilcoxon’s signed-rank tests, p \textless 0.001) of BMAA isomers were observed in TCA-amended samples (method B) compared to samples without TCA (method A). The overall range of B/A ratios was 0.67-8.25 for AEG (up to +725%) and 0.69-15.5 for DAB (up to +1450%), with absolute concentration increases in TCA-amended samples of up to +15,000 ng L-1 for AEG and +650 ng L-1 for DAB. We also documented the trends in the occurrence of BMAA isomers for a large breadth of field-collected lakes from Brazil, Canada, France, Mexico, and the United Kingdom. Data gathered during this overarching campaign (overall, n = 390 within 45 lake sampling sites) indicated frequent detections of AEG and DAB isomers, with detection rates of 30% and 43% and maximum levels of 19,000 ng L-1 and 1100 ng L-1, respectively. In contrast, BAMA was found in less than 8% of the water samples, and BMAA was not found in any sample. These results support the analyses of free-living cyanobacteria, wherein BMAA was often reported at concentrations of 2-4 orders of magnitude lower than AEG and DAB. Seasonal measurements conducted at two bloom-impacted lakes indicated limited correlations of BMAA isomers with total microcystins or chlorophyll-a, which deserves further investigation.
%R 10.3390/toxins14040251
%U https://gwf-uwaterloo.github.io/gwf-publications/G22-22001
%U https://doi.org/10.3390/toxins14040251
%P 251
Markdown (Informal)
[Occurrence of BMAA Isomers in Bloom-Impacted Lakes and Reservoirs of Brazil, Canada, France, Mexico, and the United Kingdom](https://gwf-uwaterloo.github.io/gwf-publications/G22-22001) (Abbes et al., GWF 2022)
ACL
- Safa Abbes, Sung Vo Duy, Gabriel Munoz, Quoc Tuc Dinh, Dana F. Simon, Barry Husk, Helen M. Baulch, Brigitte Vinçon‐Leite, Nathalie Fortin, Charles W. Greer, Megan L. Larsen, Jason J. Venkiteswaran, Fernando Martínez‐Jerónimo, Alessandra Giani, Chris Lowe, Nicolas Tromas, and Sébastien Sauvé. 2022. Occurrence of BMAA Isomers in Bloom-Impacted Lakes and Reservoirs of Brazil, Canada, France, Mexico, and the United Kingdom. Toxins, Volume 14, Issue 4, 14(4):251.
