Deborah L. MacLatchy


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Reproductive status of walleye (Sander vitreus) and lake whitefish (Coregonus clupeaformis) in two large, shallow Canadian subarctic lakes
Heather Dixon, Grant Von Harrison, Andrea Lister, Deborah L. MacLatchy
Environmental Biology of Fishes, Volume 103, Issue 9

The reproductive status of walleye (Sander vitreus) and lake whitefish (Coregonus clupeaformis) is largely unstudied in the northern extent of their ranges. Tathlina Lake and Kakisa Lake are large, shallow lakes in the Northwest Territories, Canada, supporting important commercial and subsistence fisheries for these species while being threatened by climate change. Fish were sampled in both lakes across multiple years in the spring and autumn to assess differences in reproductive status in the pre- and post-spawning periods for both species. Condition factor (K), gonadosomatic index (GSI), liversomatic index (LSI), and fecundity were calculated, and plasma samples were also taken from each fish to determine levels of reproductive hormones, specifically 17β-estradiol in females, and 11-ketotestosterone in males. Significant temporal (intra- and interannual) and spatial (between lakes) variation was found for both species and both sexes for all metrics. Expected differences in hormones and indices of reproductive success between pre- and post- spawning periods were demonstrated. When compared with previously published data, a latitudinal gradient for LSI, GSI and fecundity was evident for walleye, but not for lake whitefish. The differences in the reproductive biology of lake whitefish and walleye in these two neighbouring lakes highlights limitations in the use of a reference lake approach in biomonitoring studies. The data in this study can be used and expanded upon to provide information for the sustainable management of these fish stocks for the future.


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Assessing recovery of in vitro steroid production in male rainbow darter (<i>Etheostoma caeruleum</i>) in response to municipal wastewater treatment plant infrastructure changes
Patricija Marjan, Glen J. Van Der Kraak, Deborah L. MacLatchy, Meghan Fuzzen, Leslie M. Bragg, Mark E. McMaster, Gerald R. Tetreault, Mark R. Servos
Environmental Toxicology and Chemistry, Volume 37, Issue 2

The present study examined in vitro 11-ketotestosterone and testosterone production by the testes of rainbow darter (Etheostoma caeruleum) collected from selected reference sites and downstream of 2 municipal wastewater treatment plants (MWWTPs; Waterloo and Kitchener) on the central Grand River (Ontario, Canada), over a 6-yr period (2011-2016). The main objective was to investigate if infrastructure upgrades at the Kitchener MWWTP in 2012 resulted in a recovery of this response in the post-upgrade period (2013-2016). Two supporting studies showed that the fall season is appropriate for measuring in vitro sex steroid production because it provides stable detection of steroid patterns, and that the sample handling practiced in the present study did not introduce a bias. Infrastructure upgrades of the Kitchener MWWTP resulted in significant reductions in ammonia and estrogenicity. After the upgrades, 11-ketotestosterone production by MWWTP-exposed fish increased in 2013 and it continued to recover throughout the study period of 2014 through 2016, returning to levels measured in reference fish. Testosterone production was less sensitive and it lacked consistency. The Waterloo MWWTP underwent some minor upgrades but the level of ammonia and estrogenicity remained variable over time. The production of 11-ketotestosterone and testosterone in rainbow darter below the Waterloo MWWTP was variable and without a clear recovery pattern over the course of the present study. The results of the present study demonstrated that measuring production of sex steroids (especially 11-ketotestosterone) over multiple years can be relevant for assessing responses in fish to environmental changes such as those resulting from major infrastructure upgrades. Environ Toxicol Chem 2018;37:501-514. © 2017 SETAC.