Microcystins (MCs) produced by some cyanobacteria can cause toxicity in animals and humans. In recent years, growing evidence suggests that MCs can act as endocrine disruptors. This research systematically investigated effects of microcystin-LR (MC-LR) on endocrine organs, biosynthesis of hormones and positive/negative feedback of the endocrine system in rats. Male, Sprague-Dawley rats were acutely administrated MC-LR by a single intraperitoneal injection at doses of 45, 67.5 or 90 μg MC-LR/kg body mass (bm), and then euthanized 24 h after exposure. In exposed rats, histological damage of hypothalamus, pituitary, adrenal, testis and thyroid were observed. Serum concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and corticosterone (CORT), expressions of genes and proteins for biosynthesis of hormones were lesser, which indicated an overall suppression of the hypothalamus-pituitary-adrenal (HPA) axis. Along the hypothalamus-pituitary-gonadal (HPG) axis, lesser concentrations of gonadotropin-releasing hormone (GnRH) and testosterone (T), but greater concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2) were observed. Except for greater transcription of cyp19a1 in testes, transcriptions of genes and proteins for T and E2 biosynthesis along the HPG axis were lesser. As for the hypothalamus-pituitary-thyroid (HPT) axis, after MCs treatment, greater concentrations of thyroid-stimulating hormone (TSH), but lesser concentrations of free tri-iodothyronine (fT3) were observed in serum. Concentrations of free tetra-iodothyronine (fT4) were greater in rats dosed with 45 μg MCs/kg, bm, but lesser in rats dosed with 67.5 or 90 μg MCs/kg, bm. Transcripts of genes for biosynthesis of hormones and receptors along the HPT axis and expressions of proteins for biosynthesis of tetra-iodothyronine (T4) and tri-iodothyronine (T3) in thyroid were significantly altered. Cross-talk among the HPA, HPG and HPT axes probably occurred. It was concluded that MCs caused an imbalance of positive and negative feedback of hormonal regulatory axes, blocked biosynthesis of key hormones and exhibited endocrine-disrupting effects.

Microcystins (MCs) produced by some cyanobacteria can cause toxicity in animals and humans. In recent years, growing evidence suggests that MCs can act as endocrine disruptors. This research systematically investigated effects of microcystin-LR (MC-LR) on endocrine organs, biosynthesis of hormones and positive/negative feedback of the endocrine system in rats. Male, Sprague-Dawley rats were acutely administrated MC-LR by a single intraperitoneal injection at doses of 45, 67.5 or 90 μg MC-LR/kg body mass (bm), and then euthanized 24 h after exposure. In exposed rats, histological damage of hypothalamus, pituitary, adrenal, testis and thyroid were observed. Serum concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and corticosterone (CORT), expressions of genes and proteins for biosynthesis of hormones were lesser, which indicated an overall suppression of the hypothalamus-pituitary-adrenal (HPA) axis. Along the hypothalamus-pituitary-gonadal (HPG) axis, lesser concentrations of gonadotropin-releasing hormone (GnRH) and testosterone (T), but greater concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2) were observed. Except for greater transcription of cyp19a1 in testes, transcriptions of genes and proteins for T and E2 biosynthesis along the HPG axis were lesser. As for the hypothalamus-pituitary-thyroid (HPT) axis, after MCs treatment, greater concentrations of thyroid-stimulating hormone (TSH), but lesser concentrations of free tri-iodothyronine (fT3) were observed in serum. Concentrations of free tetra-iodothyronine (fT4) were greater in rats dosed with 45 μg MCs/kg, bm, but lesser in rats dosed with 67.5 or 90 μg MCs/kg, bm. Transcripts of genes for biosynthesis of hormones and receptors along the HPT axis and expressions of proteins for biosynthesis of tetra-iodothyronine (T4) and tri-iodothyronine (T3) in thyroid were significantly altered. Cross-talk among the HPA, HPG and HPT axes probably occurred. It was concluded that MCs caused an imbalance of positive and negative feedback of hormonal regulatory axes, blocked biosynthesis of key hormones and exhibited endocrine-disrupting effects.

Health risks of chronic exposure to microcystins (MCs), a family of aquatic contaminants produced mainly by cyanobacteria, are critical yet unsolved problems. Despite a few epidemiological studies, the metabolic profiles of humans exposed to MCs remain unknown, hindering the deep understanding of the molecular toxicity mechanisms. Here, sensitive nuclear magnetic resonance (NMR)- and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics were applied to investigate the serum metabolic profiles of humans living near Lake Chao, where toxic cyanobacterial blooms occur annually. MCs were positively detected in 92 of 144 sera by ultra-high-pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with a median concentration of 0.016 μg/L. The estimated daily intake (0.15-0.27 μg MC-LReq/day) was less than the tolerable daily intake (TDI, 2.4 μg MC-LR for 60 kg adults) recommended by the World Health Organization (WHO). Obvious disruptions of the amino acid metabolism were confirmed and played important roles in renal impairments associated with serum MC burdens. Chronic oral exposure of mice to 30 μg MC-LR/kg body mass, which is less than the no observed adverse effect level, also led to obvious renal lesions and metabolic dysfunction. These observations provide the first evidence of metabolic disturbance of humans exposed to MCs and indicate that the WHO's TDI value determined traditionally should be lessened to protect human health effectively.

Abstract. To assess the hydroclimatic risks posed by climate change in western Canada, this study conducted a retrospective simulation (CTL) and a pseudo-global warming (PGW) dynamical downscaling of future warming projection under RCP8.5 from an ensemble of CMIP5 climate model projections using a convection-permitting 4-km Weather Research Forecasting (WRF) model. The convection-permitting resolution of the model avoids the error-prone convection parameterization by explicitly resolving cumulus plumes. The evaluation of surface air temperature by the retrospective simulation WRF-CTL against a gridded observation ANUSPLIN shows that WRF simulation of daily mean temperature agrees well with ANUSPLIN temperature in terms of the geographical distribution of cold biases east of the Canadian Rockies, especially in spring. Compared with the observed precipitation from ANUSPLIN and CaPA, the WRF-CTL simulation captures the main pattern of distribution, but with a wet bias seen in higher precipitation near the British Columbia coast in winter and over the immediate region on the lee side of the Canadian Rockies. The PGW simulation shows more warming than CTL, especially over the polar region in the northeast, during the cold season, and in daily minimum temperature. Precipitation changes in PGW over CTL vary with the seasons: In spring and late fall for both basins, precipitation is shown to increase, whereas in summer in the Saskatchewan River Basin, it either shows no increase or decreases, with less summer precipitation shown in PGW than in CTL for some parts of the Prairies. This seasonal difference in precipitation change suggests that in summer the Canadian Prairies and the southern Boreal Forest biomes will likely see a slight decline in precipitation minus evapotranspiration, which might impact soil moisture for farming and forest fires. With almost no increase in summer precipitation and much more evapotranspiration in PGW than in CTL, the water availability during the growing season will be challenging for the Canadian Prairies. WRF-PGW shows an increase of high-intensity precipitation events and shifts the distribution of precipitation events toward more extremely intensive events in all seasons, as current moderate events become extreme events with more vapor loading, especially in summer. Due to this shift in precipitation intensity to the higher end in the PGW simulation, the seemingly moderate increase in the total amount of precipitation in summer for both the Mackenzie and Saskatchewan river basins may not reflect the real change in flooding risk and water availability for agriculture. The high-resolution downscaled climate simulations provide abundant opportunities both for investigating local-scale atmospheric dynamics and for studying climate impacts in hydrology, agriculture, and ecosystems. The change in the probability distribution of precipitation intensity also calls for innovative bias-correction methods to be developed for the application of the dataset when bias-correction is required.

Uncertainties in representing land–atmosphere interactions can substantially influence regional climate simulations. Among these uncertainties, the surface exchange coefficient Ch is a critical parameter, controlling the total energy transported from the land surface to the atmosphere. Although it directly impacts the coupling strength between the surface and atmosphere, it has not been properly evaluated for regional climate models. This study assesses the representation of surface coupling strength in a stand-alone Noah-MP land surface model and in coupled 4-km Weather Research and Forecasting (WRF) model simulations. The data collected at eight FLUXNET sites of the Canadian Carbon Program and seven AMRIFLUX sites are used to evaluate the offline Noah-MP simulations. Nine of these FLUXNET sites are used for the evaluation of the coupled WRF simulations. These sites are categorized into three land use types: grassland, cropland, and forest. The surface exchange coefficients derived using three formulations in Noah-MP simulations are compared to those calculated from observations. Then, the default Czil  = 0 and new canopy-height dependent Czil are used in coupled WRF simulations over the spring and summer in 2006 to compare their effects on surface heat flux, temperature, and precipitation. When the new canopy-height dependent Czil scheme is used, the simulated Ch exchange coefficient agrees better with observation and improves the daily maximum air temperature and heat flux simulation over grassland and cropland in the US Great Plains. Over grassland, the modeled Ch shows a different diurnal cycle than that for observed Ch, which makes WRF lag behind the observed diurnal cycle of sensible heat flux and temperature. The difference in precipitation between the two schemes is not as clear as the temperature difference because the impact of changing Ch is not local.

Abstract. Climate change poses great risks to western Canada's ecosystem and socioeconomical development. To assess these hydroclimatic risks under high-end emission scenario RCP8.5, this study used the Weather Research Forecasting (WRF) model at a convection-permitting (CP) 4 km resolution to dynamically downscale the mean projection of a 19-member CMIP5 ensemble by the end of the 21st century. The CP simulations include a retrospective simulation (CTL, 2000–2015) for verification forced by ERA-Interim and a pseudo-global warming (PGW) for climate change projection forced with climate change forcing (2071–2100 to 1976–2005) from CMIP5 ensemble added on ERA-Interim. The retrospective WRF-CTL's surface air temperature simulation was evaluated against Canadian daily analysis ANUSPLIN, showing good agreements in the geographical distribution with cold biases east of the Canadian Rockies, especially in spring. WRF-CTL captures the main pattern of observed precipitation distribution from CaPA and ANUSPLIN but shows a wet bias near the British Columbia coast in winter and over the immediate region on the lee side of the Canadian Rockies. The WRF-PGW simulation shows significant warming relative to CTL, especially over the polar region in the northeast during the cold season, and in daily minimum temperature. Precipitation changes in PGW over CTL vary with the seasons: in spring and late autumn precipitation increases in most areas, whereas in summer in the Saskatchewan River basin and southern Canadian Prairies, the precipitation change is negligible or decreased slightly. With almost no increase in precipitation and much more evapotranspiration in the future, the water availability during the growing season will be challenging for the Canadian Prairies. The WRF-PGW projected warming is less than that by the CMIP5 ensemble in all seasons. The CMIP5 ensemble projects a 10 %–20 % decrease in summer precipitation over the Canadian Prairies and generally agrees with WRF-PGW except for regions with significant terrain. This difference may be due to the much higher resolution of WRF being able to more faithfully represent small-scale summer convection and orographic lifting due to steep terrain. WRF-PGW shows an increase in high-intensity precipitation events and shifts the distribution of precipitation events toward more extremely intensive events in all seasons. Due to this shift in precipitation intensity to the higher end in the PGW simulation, the seemingly moderate increase in the total amount of precipitation in summer east of the Canadian Rockies may underestimate the increase in flooding risk and water shortage for agriculture. The change in the probability distribution of precipitation intensity also calls for innovative bias-correction methods to be developed for the application of the dataset when bias correction is required. High-quality meteorological observation over the region is needed for both forcing high-resolution climate simulation and conducting verification. The high-resolution downscaled climate simulations provide abundant opportunities both for investigating local-scale atmospheric dynamics and for studying climate impacts on hydrology, agriculture, and ecosystems.

Abstract A devastating, flood-producing rainstorm occurred over southern Alberta, Canada, from 19 to 22 June 2013. The long-lived, heavy rainfall event was a result of complex interplays between topographic, synoptic, and convective processes that rendered an accurate simulation of this event a challenging task. In this study, the Weather Research and Forecasting (WRF) Model was used to simulate this event and was validated against several observation datasets. Both the timing and location of the model precipitation agree closely with the observations, indicating that the WRF Model is capable of reproducing this type of severe event. Sensitivity tests with different microphysics schemes were conducted and evaluated using equitable threat and bias frequency scores. The WRF double-moment 6-class microphysics scheme (WDM6) generally performed better when compared with other schemes. The application of a conventional convective/stratiform separation algorithm shows that convective activity was dominant during the early stages, then evolved into predominantly stratiform precipitation later in the event. The HYSPLIT back-trajectory analysis and regional water budget assessments using WRF simulation output suggest that the moisture for the precipitation was mainly from recycling antecedent soil moisture through evaporation and evapotranspiration over the Canadian Prairies and the U.S. Great Plains. This analysis also shows that a small fraction of the moisture can be traced back to the northeastern Pacific, and direct uptake from the Gulf of Mexico was not a significant source in this event.